Cephalosporin C production in a stirred tank reactor |
| |
Authors: | T Herold T Bayer K Schügerl |
| |
Institution: | (1) Sartorius GmbH, Göttingen;(2) Hoechst AG, Frankfurt, D-6000 Frankfurt, Federal Republic of Germany;(3) Institut für Technische Chemie, Universität Hannover, Callinstr. 3, D-3000 Hannover, Federal Republic of Germany |
| |
Abstract: | Summary Cephalosporin C was produced with the moldCephalosporium acremonium in a 20 1 stirred tank reactor with 100 kg/m3 peanut flour in fed-batch operation. The growth and product formation was followed by on-line analysis of the broth composition. The cell concentration was estimated from the RNA-content of the cells. By optimization of the fed-batch operation and by increasing the phosphate content in the broth, a final cephalosporin C concentration of 12 kg/m3 was attained.Nomenclature CPC
cephalosphorin C
- DAC
deacetylcephalosporin C
- DAOC
deacetoxycephalosporin C
-
k
L
a
volumetric mass transfer coefficient
- MMBS
2-Hydroxy-4-methylmercaptobutyric acid
- PABAH
p-Hydroxybenzoicacidhydrazid
- RNA
ribonucleic acid
- RQ
respiratory quotient
-
oxygen transfer rate
-
CO2-production rate
-
t
fermentation time |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|