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Alignment of clonedamiE gene ofPseudomonas aeruginosa with theN-terminal sequence of amidase |
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| Authors: | P H Clarke R E Drew C Turberville W J Brammar R P Ambler A D Auffret |
| Institution: | (1) Department of Biochemistry, University College London, WC1E 6BT London, U.K.;(2) Department of Biochemistry, University of Leicester, LE1 7RH Leicester, U.K.;(3) Department of Molecular Biology, University of Edinburgh, EH9 3JR Edinburgh, U.K.;(4) Present address: Department of Biochemistry, University of Leeds, LS2 9JT Leeds, U.K. |
| Abstract: | A restriction enzyme map was constructed for 5.1-kb fragment of Pseudomonas aeruginosa DNA inserted into plasmid pBR322. Restriction enzyme sites were matched to the N-terminal amino acid sequence of amidase to obtain alignment of the amiE gene within the cloned fragment. |
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