A cross-sectional study of umbilical cord blood donor profiles and their influence on umbilical cord blood collection in a Brazilian hospital |
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| Authors: | Valéria Santos Sandra Marti Luciana Ribeiro Andreza Alice Feitosa Conti Fabiana Barros Sonia Maria |
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| Affiliation: | 1. Umbilical Cord Blood Bank, Hospital Israelita Albert Einstein, São Paulo, Brazil;2. Instituto Israelita de Ensino e Pesquisa Albert Einstein, São Paulo, Brazil;3. Universidade Federal de São Paulo, São Paulo, Brazil;1. Department of Gastroenterology and Hepatology;2. Department of Immunohematology and Blood Transfusion;3. Department of Nephrology, Leiden University, Medical Center, Leiden, the Netherlands;1. Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, USA;2. Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, P. R. China;2. Current address: Department of Hepatobiliary Surgery, The Affiliated Hospital of Guiyang Medical College, Guiyang, P. R. China;1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic;2. Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Charles University, Prague, Czech Republic;1. Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children''s Hospital, Houston, Texas, USA;2. Department of Medicine, Section of Hematology/Oncology, Baylor College of Medicine, Houston, Texas, USA;3. MaxCyte Inc., Gaithersburg, Maryland, USA;1. Laboratory of Advanced Cellular Therapies, Department of Hematology, S. Bortolo Hospital, Vicenza, Italy (formerly at Cell Therapy Unit, Cardiocentro Ticino, Lugano, Switzerland);2. Blood Bank and Cell Therapy Unit, Department of Hemato-Oncology, Children''s Hospital A. Meyer, Florence, Italy;3. Cell Therapy Unit, Cardiocentro Ticino, Lugano, Switzerland;1. Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic;2. Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, Prague, Czech Republic |
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| Abstract: | Background aimsUmbilical cord blood (UCB) cells are a new alternative to bone marrow source for hematopoietic stem cell transplantation and their use has increased in the last decade. Thus efforts are being made to improve the umbilical cord blood unit's quality. Besides compatibility, other factors, such as the total nucleated cell (TNC) count and the percentage of CD34+ cells in the product, are very important for a successful transplant outcome. Our aim was to describe our donor population and assess the best cord blood collection technique at Hospital Israelita Albert Einstein's cord blood bank (São Paulo, Brazil).MethodsThis was a retrospective study in which all analyses were performed simultaneously. A Student's t-test was used for qualitative variables for non-matched samples. For qualitative analyses, we used either the chi-square test or the exact Fisher's test.ResultsThe stratification of the population characteristics allowed us to determine which ones had an impact on unit volume, TNC count and percentage of CD34+ cells. A significant correlation was observed between donor characteristics and the quality of UCB units as related to maternal and gestational age, type of pregnancy, route of delivery, cord blood collection technique and birth weight.ConclusionsWe found that cord blood collection technique and newborn weight were significantly correlated with the TNC content. The collection technique used at our institution significantly improved the UCB unit volume and consequently the TNC count. Some findings, such as the impact of maternal age and newborn weight, have led us to re-evaluate our protocol in order to achieve better results. |
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