Identification and expression analysis of EST-based genes in the bud of Lycoris longituba |
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| Authors: | Cui Yonglan Zhang Xinye Zhou Yan Yu Hong Tao Lin Zhang Lu Zhou Jian Zhuge Qiang Cai Youming Huang Minren |
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| Affiliation: | 1.Key Laboratory of Tree Genetic Engineering of Nanjing Forestry University, Nanjing 210037, China;2.Hangzhou Genomics Institute/James D. Watson Institute of Genome Sciences, Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310008, China;3.Department of Forestry, Jiangxi Agriculture University, Nanchang 330045, China;4.Shanghai Municipal Agricultural Commission, Shanghai 200003, China |
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| Abstract: | To obtain a primary overview of gene diversity and expression pattern in Lycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were se-quenced and 4,687 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 967 contigs and 1,343 singlets were obtained. Blast search showed that 179 contigs and 227 singlets (totally 1,066 ESTs) had homologues in GenBank and 3,621 ESTs were novel. |
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| Keywords: | Lycoris longituba EST |
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