Melatonin inhibits the proliferation of retinal pigment epithelial (RPE) cells in vitro |
| |
Authors: | Hing-Sing Yu Veronica Hernandez Mark Haywood Corinne G Wong |
| |
Institution: | (1) Biorhythm Research Laboratory, Division of Mathematics, Computer Science, and Statistics, The University of Texas at San Antonio, 78249 San Antonio, Texas;(2) Department of Ophthalmology, The University of Texas Health Science Center at San Antonio, 78284 San Antonio, Texas;(3) Department of Ophthalmology, The University of California at Irvine, 92717 Irvine, California |
| |
Abstract: | Summary The possible antiproliferative effect of melatonin on retinal pigment epithelial (RPE) cells in vitro was investigated. Bovine
RPE cells cultured in Ham’s F12 medium supplemented with 10% fetal bovine serum had a nuclear density of 73.6 ± 6.1 nuclei/mm2 at 72 h after seeding. The nuclear density at this time-point was doubled if either 50 or 100 ng/ml human epidermal growth
factors (hEGF) was added to the culture medium. When these hEGF-stimulated cells were treated with melatonin from 10 to 500
pg/ml, the proliferation was suppressed with a dose-response relationship. At 250 and 500 pg/ml melatonin, the nuclear densities
of the melatonin-treated cells were similar to those of the control cells. Using mitotically active SV-40 transformed human
fetal RPE cells cultured in a serum-free medium, melatonin was also shown to be antiproliferative. In the presence of 500
pg/ml melatonin, the proliferation of these cells was inhibited to 77% as compared to the control. These results were further
supported by the reduced H3]thymidine uptake in the melatonin-treated cells. We propose that melatonin, at physiologic concentrations, has an antiproliferative
effect, and that cultured RPE cells stimulated to proliferate by either hEGF treatment or SV-40 transfection are responsive
to melatonin. Melatonin may either inhibit mitosis in actively dividing cells or modulate hEGF action. |
| |
Keywords: | melatonin EGF human bovine epithelial cells cell culture |
本文献已被 SpringerLink 等数据库收录! |
|