Isolation of the transposable element hupfer from the entomopathogenic fungus Beauveria bassiana by insertion mutagenesis of the nitrate reductase structural gene |
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| Institution: | (1) I.N.R.A., Station de Recherches de Lutte Biologique, La Minière, F-78285 Guyancourt Cedex, France, FR;(2) C.N.R.S., Laboratoire de Biologie et Génétique Evolutives, F-91198 Gif-sur-Yvette Cedex, France, FR;(3) Université Paris XI, Laboratoire de Cryptogamie, Bat. 400, F-91405 Orsay Cedex, France, FR;(4) I.N.R.A., Unité de Phytopharmacie et Médiateurs Chimiques, route de Saint-Cyr, F-78026 Versailles Cedex, France e-mail: maurer@versailles.inra.fr, FR |
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| Abstract: | A transposable element has been isolated from the entomopathogenic fungus Beauveria bassiana by trapping it in the nitrate reductase structural gene, which has been cloned from this species. The element had inserted
in the first exon of the nia gene and appeared to have duplicated the sequence TA at the site of insertion. It was 3336 bp long with 30-bp imperfect, inverted,
terminal repeats. The element, called hupfer, contained an open reading frame encoding a 321-amino acid protein similar to the IS630- or mariner-Tc1-like transposases, and a residual sequence of about 2 kb which was not significantly similar to any published sequence. There
are fewer than five copies of this transposable element present per genome in the fungus.
Received: 12 February 1997 / Accepted: 2 May 1997 |
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| Keywords: | Transposon Filamentous fungus Tc1/mariner IS |
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