Construction and characterization of a bacterial artificial chromosome library of the causal agent of Black Sigatoka fungal leaf spot disease of banana and plantain, <Emphasis Type="Italic">Mycosphaerella fijiensis</Emphasis> |
| |
Authors: | Blondy Canto-Canché Diana Karina Guillén-Maldonado Leticia Peraza-Echeverría Laura Conde-Ferráez Andrew James-Kay |
| |
Institution: | (1) Centro de Investigación Científica de Yucatán, Unidad de Biotecnología, Calle 43 No. 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, México |
| |
Abstract: | A bacterial artificial chromosome library of the causal agent of the Black Sigatoka leaf spot disease of banana and plantain,
Mycosphaerella fijiensis, has been constructed using a non-sphaeroplasting technique and characterized using both homologous and heterologous probes.
After first and a second size selection of PFGE-fractionated DNA, a ligation was obtained using a 1:4 molar ratio (insert:vector).
One hundred random clones were analyzed, and the mean insert size was estimated to be 90 kb. The range of the insert sizes
was between 40 and 160 kb. The highest percentage of inserts belonged to the range between 80 and 100 kb; 32% of the inserts
had 2 or 3 internal NotI sites. This library consists of 1920 clones, if the genomic size is at least 35 Mb, then this represents 4.9× genome equivalents,
which was supported by hybridization results with homologous and heterologous probes.
Blondy Canto-Canché and Diana Karina Guillén-Maldonado contributed equally to this work and should be regarded as co-first
authors. |
| |
Keywords: | Mycosphaerella fijiensis Black Sigatoka BAC library |
本文献已被 PubMed SpringerLink 等数据库收录! |
|