| 氧化亚铁硫杆菌分离复壮及固定化的研究 |
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| 引用本文： | 邸进申,赵新巧,耿冰.氧化亚铁硫杆菌分离复壮及固定化的研究[J].微生物学报,2003,43(4):487-491. |
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| 作者姓名： | 邸进申 赵新巧 耿冰 |
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| 作者单位： | 河北工业大学化工学院生物工程系,天津,300130 |
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| 基金项目： | 河北工业大学《2 1 1工程》资助项目~~ |
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| 摘 要： | 用稀释涂布平板法从已退化的氧化亚铁硫杆菌（Thiobacillus ferrooxidans）菌液中分离出氧化活性较高、生命力强的氧化亚铁硫杆菌T1。以H2软性填料作为氧化亚铁硫杆菌的固定化载体，构建了固定床生物反应器。考察了固定床生物反应器氧化Fe2+的情况：Fe2+最大氧化速率达7.67g/(L·h)。并对固定床生物反应器运行过程中在载体表面形成的沉淀物进行了研究，通过X衍射证明此沉淀物为黄钾铁矾［Kfe3(SO4)2(OH)6］。
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| 关 键 词： | 氧化亚铁硫杆菌，固定化，载体，固定床生物反应器 |
| 文章编号： | 0001-6209(2003)04-0487-05 |
Study on the Rejuvenating by Isolation and the Immobilization of Thiobacillus Ferrooxidans |
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| Di Jinshen,Zhao Xinqiao Geng Bing.Study on the Rejuvenating by Isolation and the Immobilization of Thiobacillus Ferrooxidans[J].Acta Microbiologica Sinica,2003,43(4):487-491. |
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| Authors: | Di Jinshen Zhao Xinqiao Geng Bing |
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| Institution: | School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China. |
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| Abstract: | A strain of Thiobacillus ferrooxidation T1 with stronger oxidation ability and more exuberant vitality was isolated from the liquid with regressive Thiobacillus ferrooxidans by method of dilution butteron on plate. A fixed-bed bioreactor was constructed by immobilized cells of Thiobacillus ferrooxidans with H-2 carriers. In the state of bioreactor operation, a maximum Fe2+ oxidation rate of 7.67gFe2+] x h(-1) x L(-1) was attained. We studied the precipitates formed on the surface of the carriers during the operation of the bioreactor. By X-ray diffraction analyses we proved the precipitates were jarosite. |
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| Keywords: | Thiobacillus ferrooxidans Immobilization Carriers Fixed-bed bioreactor |
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