Comparative effect of FGF2, synthetic peptides 1-28 N-POMC and ACTH on proliferation in rat adrenal cell primary cultures |
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Authors: | Gabriele E Mattos Jacqueline F Jacysyn Gustavo P Amarante-Mendes Claudimara Ferini Pacicco Lotfi |
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Institution: | 1.Department of Anatomy, Institute of Biomedical Sciences,University of S?o Paulo,S?o Paulo,Brazil;2.School of Medicine - LIM62,University of S?o Paulo,S?o Paulo,Brazil;3.Department of Immunology, Institute of Biomedical Sciences,University of S?o Paulo,S?o Paulo,Brazil;4.Departamento de Anatomia, Instituto de Ciências Biomédicas,Universidade de S?o Paulo,S?o Paulo,Brazil |
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Abstract: | There is evidence that pro-opiomelanocortin (POMC)-derived peptides other than adrenocorticotropic hormone (ACTH) have a role
in adrenal cell proliferation. We compared the activity of synthetic rat N-terminal POMC fragment 1-28 with disulfide bridges
(N-POMCw) and without disulfide bridges (N-POMCw/o), with the activity of fibroblast growth factor (FGF2), a widely studied adrenal growth factor, and ACTH, in well-characterized
pure cultures of both isolated adrenal Glomerulosa (G) and Fasciculata/Reticularis (F/R) cells. Three days of FGF2-treatment
had a proliferative effect similar to serum, and synthetic peptide N-POMCw induced proliferation more efficiently than N-POMCw/o. Moreover, both induced proliferation via the ERK1/2 pathway. In contrast, sustained ACTH treatment decreased proliferation
and viability through apoptosis induction, but not necrosis, and independently of PKA and PKC pathways. Further elucidation
of 1-28 POMC signal transduction is of interest, and primary cultures of adrenal cells were found to be useful for examining
the trophic activity of this peptide. |
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