| 桦褐孔菌三萜化合物提取工艺的优选 |
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| 引用本文： | 潘春丽,CHA Yong,张鑫,YU Xiao-bin. 桦褐孔菌三萜化合物提取工艺的优选[J]. 生物加工过程, 2008, 6(4): 36-39 |
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| 作者姓名： | 潘春丽 CHA Yong 张鑫 YU Xiao-bin |
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| 作者单位： | 江南大学,生物工程学院,工业生物技术教育部重点实验室,无锡,214122 |
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| 基金项目： | 教育部长江学者和创新团队发展计划资助项目 |
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| 摘 要： | 为了提高桦褐孔菌三萜化合物的产量，对其三萜化合物的提取工艺进行了初步优选。试验以桦褐孔菌发酵菌丝体为材料，齐墩果酸为标准品，用香草醛比色法测桦褐孔菌三萜化合物含量。通过单因素实验考察了不同提取剂、提取剂用量、提取温度、提取时间对桦褐孔菌三萜化合物提取的影响。结果表明，提取的最佳工艺为：以异丙醇为提取溶剂，用量为7倍，最佳提取温度为80℃，提取时间2h，在此条件下三萜化合物占菌丝体干质量的9．03％。该工艺的三萜桦褐物的提取率高，可用于桦褐孔菌中三萜化合物的提取。
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| 关 键 词： | 桦褐孔菌 三萜 提取工艺 |
Extraction process of triterpenoids from Inonotus obliquus |
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| PAN Chun-li,CHA Yong,ZHANG Xin,YU Xiao-bin. Extraction process of triterpenoids from Inonotus obliquus[J]. Chinese Journal of Bioprocess Engineering, 2008, 6(4): 36-39 |
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| Authors: | PAN Chun-li CHA Yong ZHANG Xin YU Xiao-bin |
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| Affiliation: | ( Key Laboratory of Industrial Biotechnology, of the Ministry of Education School of Biotechnology, Jiangnan Uniwersity, Wuxi 214122, China) |
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| Abstract: | The extraction process of triterpenoids from Inonotus obliquus was investigated to increase the triterpenoids yield. The triterpenoids composition was determined by spectrometric method with oleanolic acid as a reference. The influence of solvent, extraction dosage, extracting temperature, and extracting time on the triterpenoids of yield was evaluated by single factor test. The results showed that the optimum conditions were 7 times in isopropanol and the extraction time 2 h at 80℃. The extraction rate of triterpenoids was 9.03 %. |
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| Keywords: | Inonotus obliquus triterpenoids extraction |
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