The use of PCR to monitor the population abundance of six human intestinal bacterial species in an in vitro semicontinuous culture system |
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Authors: | Rong-Fu Wang Wei-Wen Cao Warren L. Campbell Latrina Hairston Wirt Franklin Carl E. Cerniglia |
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Affiliation: | Microbiology Division, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA |
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Abstract: | Abstract Six PCR primer sets complementary to the 16S rDNAs (rRNA genes) were developed and shown to be specific for the following anaerobic bacteria: Clostridium clostridiiforme, C. perfringens, C. leptum, Bacteroides vulgatus, B. distasonis , and B. thetaiotaomicron , respectively. These primers were used for PCR to detect and monitor the bacteria in a semicontinuous culture system designed to mimic intestinal microflora in the human gastrointestinal tract. Except for C. perfringens , the five species of Bacteroides and Clostridia present in the in vitro culture system were detected by the PCR, and the titers varied from 10−2 to 10−6 dilutions. The role of azo dye reduction by these bacterial species in the system was examined and discussed. |
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Keywords: | Polymerase chain reaction Human intestinal microflora Azo dye |
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