Preparation of a covalently linked adduct between plastocyanin and cytochrome f |
| |
| Authors: | D J Davis K Hough |
| |
| Institution: | 1. Center for Neurochemistry, Rockland Research Institute Ward''s Island, New York 10035 USA;2. Institute Josef-Stefan, Department of Biochemistry Ljubljana 61000, Yugoslavia |
| |
| Abstract: | Plastocyanin can be covalently cross-linked to the monomeric cytochrome f from turnip by incubation in the presence of a water-soluble carbodiimide. The adduct between the two proteins has a molecular weight of approximately 43,000 suggesting a 1:1 stoichiometry between the two proteins of the adduct. This stoichiometry has been verified by spectral characterization of the adduct. The efficiency of the cross-linking reaction is pH dependent with a higher degree of cross-linking being observed at pH 6.5 than at pH 7.0. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
