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Highly selective affinity labeling of the primer-binding site of E. coli DNA polymerase I |
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| Authors: | R L Mitina A A Mustaev E F Zaychikov V V Khomov O I Lavrik |
| Affiliation: | Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Academy of Sciences, USSR. |
| Abstract: | Membrane-associated phosphoinositidase C activity has been identified in Dictyostelium discoideum using phosphatidylinositol 4,5-bisphosphate as exogenous substrate. Maximal activity was observed with 0.4 mM phosphatidylinositol 4,5-bisphosphate at pH 7.0. The enzyme was stimulated by micromolar concentrations of free calcium with maximal activity at 100 μM. |
| Keywords: | Phosphoinositidase C Phosphatidylinositol 4,5-bisphosphate Membrane Signal transduction Dictyostelium discoideum |
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