Differences between rodent and human cell lines in the amount of integrated DNA after transfection |
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| Authors: | J H Hoeijmakers H Odijk A Westerveld |
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| Affiliation: | 1. Programa de Pós-graduação em Ciências Biológicas, Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Universidade Estadual de Maringá, Bloco B-08, Av. Colombo 5790, Maringá, PR CEP 87020-900, Brazil;2. LaBioMMi, Departamento de Química, Universidade Federal de São Carlos, CP 676, São Carlos, SP 13.565-905, Brazil;1. Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University, Shanghai, China;2. The Fourth People''s Hospital of Wuhu, Wuhu 241002, China;3. School of Mental Health, Jining Medical University, Jining, China;4. Collaborative Innovation Center of Genetics and Development, Shanghai, China |
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| Abstract: | The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes. |
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