Characterization of thin layers of glucose oxidase |
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| Authors: | Bortoluzzi S Di Paolo M L Rigo A Vianello F Zennaro L |
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| Institution: | Department of Biological Chemistry, University of Padova. |
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| Abstract: | Glucose Oxidase (GOD) has been covalently bound to functionalized glass cover slips. The surface density of immobilized GOD molecules was measured by a method based on the amperometric determination of Flavin Adenine Dinucleotide (FAD). Atomic Force Microscopy (AFM) images, obtained in aqueous solution for the covalently bound enzyme, show a monomolecular layer of the enzyme on a functionalized glass surface. The catalytic constants were measured for the immobilized GOD and compared with those of the free enzyme. |
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