Genetic and fine structure analysis of unc-26(IV) and adjacent regions in Caenorhabditis elegans |
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Authors: | David L. Charest Denise V. Clark Michael E. Green David L. Baillie |
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Affiliation: | (1) Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, V5A 1S6 Burnaby, B.C., Canada;(2) Present address: Biomedical Research Centre, University of British Columbia, 2222 Health Sciences Mall, V6T 1W5 Vancouver, B.C., Canada;(3) Present address: Faculty of Medicine, University of British Columbia, 2194 Health Sciences Mall, V6T 1W5 Vancouver, B.C., Canada |
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Abstract: | Summary The genetic organization of unc-26(IV) and adjacent regions was studied in Caenorhabditis elegans. We constructed a fine structure genetic map of unc-26(IV), a gene that affects locomotion and pharyngeal muscle movement but not muscle structure. Eleven alleles were positioned relative to each other recombinationally and were classified according to phenotypic severity. The unc-26 gene spans at least 0.026 map units, which is exceptionally large for a C. elegans gene. All but one allele, e205, are amorphic alleles. Interestingly, e205 is hypomorphic but also suppressible by the amber suppressor sup-7. Nineteen lethal mutations in the unc-26 region were isolated and characterized. The unc-26 region is subdivided into four zones by five deficiency breakpoints. These mutations fall into 15 complementation groups. The stages of development affected by these mutations were determined. |
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Keywords: | Caenorhabditis elegans unc-26 Intragenic mapping Lethal mutations |
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