Preliminary analysis of bacterial diversity associated with the Porites coral from the Arabian sea |
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Authors: | Atya Kapley Sameera Siddiqui Krishna Misra Syed Masood Ahmad Hemant J Purohit |
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Institution: | (1) Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440 020, Maharashtra, India;(2) National Geophysical Research Institute, Uppal Road, Hyderabad, 500 007, Andhra Pradesh, India |
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Abstract: | It is widely reported that coral reefs are suffering degradation from a combination of stresses. We have previously reported
the use of genomic tools in the study of environmental impact assessment and hypothesize that monitoring the bacterial diversity
associated with a coral would indicate changes in its health before visible damage occurs. This study analyzes the bacterial
diversity associated with Porites coral collected from two sites in the Arabian Sea using culture independent techniques.
Two clone libraries were constructed from the16S rDNA amplicons and selected individual clones were partially sequenced. Retrieved
sequences were identified by BLAST analysis and indicate the presence of unidentified bacteria. Diversity index was calculated
using Shannon–Wiener index. The bacterial diversity associated with coral sample 1 gives the index of species diversity, H
1 as 1.6287, divergence from eqiprobability as 45.708% and the evenness of the sample as 54.291. For coral sample 2, the values
obtained were, H
1 as 1.97, divergence from eqiprobability as 15.11% and the evenness of the sample as 84.88. Sequences representing bacteria
related to agricultural or industrial pollution and pathogenesis were also found. Coral samples collected near a lagoon area,
showed a greater percentage of sequences representing bacteria related to human interventions, indicative of pollution. |
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Keywords: | Bacterial diversity Ecosystem disturbance Pollution effects Porites coral Sequencing 16S rDNA |
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