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Enzyme activity of cryoprotected myocardium.
Authors:L J Ramazzotto  R Carlin  R Engstrom
Affiliation:Department of Human Physiology, Fairleigh Dickinson University, School of Dentistry, 110 Fuller Place, Hackensack, New Jersey 07601 USA
Abstract:The use of in vivo intravenously infused DMSO/glycerol in Krebs-Henseleit buffer solution as a cryoprotectant in rat left ventricles was investigated. Alkaline phosphatase and creatine phosphokinase activities were monitored. Data show inhibition of alkaline phosphatase by the cryoprotectant solution. No significant differences were noted for the CPK activity indicating that it may not be affected by either freezing or the cryoprotectant or both in combination.
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