Characterization ofCampylobacter jejuni lipopolysaccharide |
| |
Authors: | Dr. Robert S. Conrad Chris Galanos |
| |
Affiliation: | (1) Department of Biochemistry and Microbiology, College of Osteopathic Medicine, Oklahoma State University, 1111 West 17th Street, 74107 Tulsa, OK, USA;(2) Max-Planck-Institute for Immunobiology, Freiburg, Germany |
| |
Abstract: | Lipopolysaccharides were isolated from dehydratedCampylobacter jejuni by combination of the phenol-chloroform-petroleum ether and phenol-water extraction techniques. Biochemical characterizations of lipopolysaccharide were performed on the two fractions of highest purity. Neutral sugar analyses detected galactose, glucose, trace amounts of mannose, and an unidentified deoxy-hexose. The primary amino sugars were galactosamine, glucosamine, and glucosamine-phosphate. Chemical analyses of other lipopolysaccharide components included phosphate, 3-deoxy-d-manno-octulosonic acid (KDO), and fatty acids. The predominant fatty acids were 3-hydroxytetradecanoic and hexadecanoic acids with lesser amounts of tetradecanoic acid. 3-Hydroxytetradecanoic acids were bound to lipid A by both amide and ester linkages. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|