Sexual development in Plasmodium berghei: the use of mitomycin C to separate infective gametocytes in vivo and ookinetes in vitro |
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| Authors: | A L Dearsly J Nicholas R E Sinden |
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| Institution: | 1. Departamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Apartado 0816-02593, República de Panamá;2. Departamento de Análisis Epidemiológico y Bioestadísticas, Instituto Conmemorativo Gorgas de Estudios de la Salud, Apartado 0816-02593, República de Panamá;3. Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud (INCIENSA), Apartado 4-2250, Tres Ríos, Cartago, Costa Rica;1. Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India;2. Department of Biological Sciences, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India |
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| Abstract: | Plasmodium berghei parasites were treated in vivo with mitomycin C. Giemsa stained smears of infected mouse blood showed that the asexual parasitaemia was immediately reduced while the immediate numbers of gametocytes remained unaffected. Mitomycin C treated gametocytes went on to produce ookinetes in culture. These ookinetes, which were shown to be infective to mosquitoes were readily purified by centrifugation onto 12% Nycodenz cushions at 1600 g for 10 min. The mechanism by which mitomycin C acts and the implications for Plasmodium are discussed. |
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