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MitoTimer probe reveals the impact of autophagy,fusion, and motility on subcellular distribution of young and old mitochondrial protein and on relative mitochondrial protein age
Authors:Andrew W Ferree  Kyle Trudeau  Eden Zik  Ilan Y Benador  Gilad Twig  Roberta A Gottlieb  Orian S Shirihai
Abstract:To study mitochondrial protein age dynamics, we targeted a time-sensitive fluorescent protein, MitoTimer, to the mitochondrial matrix. Mitochondrial age was revealed by the integrated portions of young (green) and old (red) MitoTimer protein. Mitochondrial protein age was dependent on turnover rates as pulsed synthesis, decreased import, or autophagic inhibition all increased the proportion of aged MitoTimer protein. Mitochondrial fusion promotes the distribution of young mitochondrial protein across the mitochondrial network as cells lacking essential fusion genes Mfn1 and Mfn2 displayed increased heterogeneity in mitochondrial protein age. Experiments in hippocampal neurons illustrate that the distribution of older and younger mitochondrial protein within the cell is determined by subcellular spatial organization and compartmentalization of mitochondria into neurites and soma. This effect was altered by overexpression of mitochondrial transport protein, RHOT1/MIRO1. Collectively our data show that distribution of young and old protein in the mitochondrial network is dependent on turnover, fusion, and transport.
Keywords:MitoTimer  aging  mitochondrial dynamics  autophagy  RHOT1
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