L-asparaginase production by <Emphasis Type="Italic">Streptomyces albidoflavus</Emphasis> |
| |
Authors: | K J P Narayana K G Kumar M Vijayalakshmi |
| |
Institution: | (1) Acharya Nagarjuna University, Guntur, 522 510, AP, India |
| |
Abstract: | Attempts were made to optimize the cultural conditions for the production of L-asparaginase by Streptomyces albidoflavus under submerged fermentations. Enhanced level of L-asparaginase was found in culture medium supplemented with maltose as
carbon source. Yeast extract (2%) was served as good nitrogen source for the production of L-asparaginase. The optimum pH
for enzyme production was 7.5 and temperature was 35°C. The release of L-asparaginase from the cells of S. albidoflavus was high when strain was treated with cell disrupting agents like EDTA and lysozyme. The enzyme produced by the strain was
purifi ed by ammonium sulfate, Sephadex G-100 and CM-Sephadex C-50 gel fi ltration and the molecular weight was apparently
determined as 112 kDa. |
| |
Keywords: | Streptomyces albidoflavus L-asparaginase Cell disrupting agents |
本文献已被 SpringerLink 等数据库收录! |