ESR检测大鼠肺巨噬细胞释放的活性氧自由基

用ESR捕捉技术检测大鼠AM释放的活性氧自由基的性质表明:1.PMA和BCG均能刺激AM产生较强的OH·;能刺激人末稍血白细胞释放活性氧自由基的ConA和顺铂在本实验条件下未能使AM产生活性氧自由基信号。2.经膜活性剂PMA刺激的AM所释放活性氧自由基的高峰在刺激后2min,而经颗粒性物质BCG刺激,AM释放自由基的高峰时间明显后移。3.测试体系中的AM数过多或过少都不适合捕捉ESR信号。在本实验条件下,捕捉到最高自由基信号的AM终浓度为5×10⁷AM/ml。4.测试体系中存在DETAPAC或EDTA,可使捕捉到的自由基信号明显增强。

STUDY OF THE PRODUCTION OF HYDROXYL RADICAL BY STIMULATED ALVEOLAR MACROPHAGES OF RAT WITH ESR SPECTROSCOPY

The characterizations of oxygen radicals released from alveolar macrophages (AM) of rats and related measurement conditions were observed with the technique of ESR in this experiment. The results were summarized as follows.1. Of PMA, BCG, ConA and cis-platinum, the first two stimulated AM to produce hydroxyl radical.2. AM stimulated by different agents had different kinetics of the release of the hydroxyl radical. The release peak from AM appeared at min. two after stimulating AM with PMA, with BCG the release peak delayed obviously.3. 5×10⁷AM/ml proved the fit concentration for trapping the signal of the hydroxyl radical from AM in this experiment. 4. There was a great influence of DETAPAC or EDTA on the intension of the signal.