| 烟草叶组织培养中光与激素对形态发生的调节作用 |
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| 引用本文： | 潘战生,陈暨耀,蔡怀新,张丕方,宋庆梅. 烟草叶组织培养中光与激素对形态发生的调节作用[J]. 分子细胞生物学报, 1989, 0(3) |
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| 作者姓名： | 潘战生 陈暨耀 蔡怀新 张丕方 宋庆梅 |
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| 作者单位： | 复旦大学物理系(潘战生，陈暨耀，蔡怀新)，复旦大学环境与资源生物学系(张丕方)，复旦大学材料科学研究所(宋庆梅) |
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| 摘 要： | 我们在烟草叶的组织培养中,研究了光与激素对形态发生所起调节作用的相互关系。在培养基中细胞分裂素与生长素的浓度比例都取得最佳值,而浓度绝对值则取两组不同数值。在这两组实验条件下,分别考察了不同波段,不同强度的光照射对于不定芽的发生和愈伤组织增重的影响。用不同波段的光照射,改变光强,光促进生长的效应都有一极大值。用蓝光照射,极大值的数值最高。激素浓度低时光的效应较大;浓度高时,除蓝光外,其他波段的光效应都很微弱。
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| 关 键 词： | 光照作用 形态发生 组织培养 烟草。 |
THE REGULATION EFFECT OF LIGHT AND HORMONES ON MORPHOGENESIS IN TOBACCO LEAF TISSUE CULTURES |
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| Pen Zhan-sheng Chen Ji-yao Cai Huai-xin. THE REGULATION EFFECT OF LIGHT AND HORMONES ON MORPHOGENESIS IN TOBACCO LEAF TISSUE CULTURES[J]. Journal of Molecular Cell Biology, 1989, 0(3) |
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| Authors: | Pen Zhan-sheng Chen Ji-yao Cai Huai-xin |
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| Abstract: | The interaction between regulation effectof light irradiation and those of hormones onmorphogenesis in tobacco leaf tissues has beenstudied.Two sets of experiments were perfor-med,in which NAA and BA concentrationswere kept at the optimum ratio but withdifferent absolute values.In each of them;the effects of light irradiations with differentwavebands and intensities on shoot initiationand callus growth were observed.For bothsets of samples,with light of different wave-bands,a maximum of response is reached byvarying the intensities and highest value ofthis maximum is attained with blue light.But light effect on samples with lower hor-mone concentration are greater than that withhigher hormone concentration.In the lattercase,only the blue light has a considerableeffect,while those of light in other wave-bands are not apparent. |
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| Keywords: | Light effect.Morphogenesis.Tissue culture.Tobacco. |
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