The nature of the complexes formed between the Int protein and DNA |
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| Authors: | Daniel Hamilton Robert Yuan Yoshiko Kikuchi |
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| Institution: | 1. Cancer Biology Program NCI Frederick Cancer Research Center P.O. Box B Frederick, Md 21701, U.S.A.;2. Laboratory of Neurochemistry National Institute of Mental Health, Bethesda, Md 20014, U.S.A. |
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| Abstract: | The λ Int protein interacts with DNA to form highly condensed complexes of a non-specific nature. Complexes formed with DNA carrying the bacterial attachment site or lacking any attachment site are dissociated by incubation with heparin or single-stranded DNA. Complexes formed with DNA carrying the phage attachment site are converted to simple complexes by these treatments, and the bound Int molecules are localized at the phage attachment site. Int is present in these complexes as an oligomer. |
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