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Identification of a fatty acid ?6-desaturase gene from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5 |
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| Authors: | Ruijiao Zhang Yuanmin Zhu Liang Ren Pengpeng Zhou Jingrong Hu Longjiang Yu |
| Institution: | 1. Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China 2. Key Laboratory of Molecular Biophysics Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, China 3. Wuhan Institute of Biotechnology, Wuhan, 430075, China |
| Abstract: | A new full-length cDNA (PsD6) putatively encoding a ?6-desaturase was cloned from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5. PsD6 contained an open reading frame of 1380 bp encoding a protein of 459 amino acids. The deduced amino acid sequence showed high similarity to those of other ?6-desaturases. A recombinant vector expressing PsD6 (pPIC3.5K-D6) was constructed and transformed into Pichia pastoris GS115. The heterologous expressed PsD6 in P. pastoris desaturated linoleic acid to γ-linolenic acid but not desaturated α-linolenic acid to stearidonic acid. The results indicated that PsD6 was a fatty acid ?6-desaturase and it had a substrate specificity for linoleic acid. |
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