Cystein-proteinase localization in osteoclasts: An immunocytochemical study |
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Authors: | Takahisa Sasaki Eriko Ueno-Matsuda |
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Institution: | (1) The Second Department of Oral Anatomy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, 142 Tokyo, Japan;(2) the Department of Pediatric Dentistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, 142 Tokyo, Japan |
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Abstract: | Summary Cysteine-proteinases such as cathepsin B and G were localized in rat osteoclasts, by an indirect protein A-immunogold labeling technique, on post-embedded ultrathin sections. In osteoclasts, specific immunogold labeling of both anti-cathepsin B and G was localized in Golgi vesicles, lysosomes, pale vacuoles of various sizes, and the extracellular canals of ruffled borders; no immunoreactivity was seen in the cytoplasmic matrix, mitchondria, cisterns of the rough endoplasmic reticulum, or nuclei. The presence of immunolabeling of cathepsins in osteoclasts and in the subosteoclastic compartment suggests that these enzymes are involved in the extracellular degradation of collagen and other noncollagenous bone matrix proteins. |
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Keywords: | Bone resorption Osteoclast Cysteine proteinase Immunocytochemistry Rats (Sprague Dawley) |
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