Inhibition of gastrulation in Xenopus embryos by an antibody against a cathepsin L-like protease |
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Authors: | Shohei Miyata Toshiyuki Kubo |
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Affiliation: | Department of Chemistry, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo 156, Japan.;Life Science Institute, Sophia University, Chiyoda-ku, Tokyo 102, Japan. |
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Abstract: | An antibody against cathepsin L-like protease (AACLP) was injected into one cell of 2-celled Xenopus embryos. The blastopores of AACLP-injected embryos either did not invaginate or failed to complete invagination. As a result of this failure to complete gastrulation, the body axes could not form normally and tail bud stage embryos were bent dorsally. Embryos injected with a control antibody (CA) developed normally through the tadpole stage. Mesodermal induction was not inhibited in embryos exhibiting this AACLP-induced gastrulation defect, but the mesodermal structure of these embryos was organized incorrectly due to the defective gastrulation during the early stages. |
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Keywords: | antibody cathepsin-L gastrulation Xenopus embryos |
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