Continuous production of diatom Entomoneis sp. in mechanically stirred tank and flat-panel airlift photobioreactors |
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| Authors: | Thunyaporn Viriyayingsiri Pantaporn Sittplangkoon Sorawit Powtongsook |
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| Affiliation: | 1. Biochemical Engineering Research Unit for Value Adding of Bioresource, Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand;2. Department of Environmental Science (Inter-Disciplinary Program), Graduate School, Chulalongkorn University, Bangkok, Thailand;3. Center of Excellence in Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;4. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand |
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| Abstract: | Continuous production of diatom Entomonies sp. was performed in mechanically stirred tank and flat-panel airlift photobioreactors (FPAP). The maximum specific growth rate of diatom from the batch experiment was 0.98 d?1. A series of dilution rate and macronutrient concentration adjustments were performed in a stirred tank photobioreactor and found that the dilution rate ranged from 0.7 to 0.8 d?1 and modified F/2 growth media containing nitrate at 3.09?mg N/L, phosphate at 2.24?mg P/L, and silicate at 11.91?mg Si/L yielded the maximum cell number density. Finally, the continuous cultivation of Entomonies sp. was conducted in FPAP using the optimal conditions determined earlier, resulting in the maximum cell number density of 19.69?×?104 cells/mL, which was approximately 47 and 73% increase from the result using the stirred tank photobioreactor fed with modified and standard F/2 growth media, respectively. |
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| Keywords: | Airlift diatom Entomoneis flat panel microalgae photobioreactor |
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