Cloning and characterization of 4.5S and 5S RNA genes in tobacco chloroplasts |
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Authors: | F Takaiwa M Sugiura |
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Institution: | National Institute of Genetics, Mishima, Shizuoka-ken 411 Japan |
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Abstract: | Tobacco chloroplast 4.5S and 5S RNAs were shown to hybridize with a 0.9 . 10(6) dalton EcoRI fragment of tobacco chloroplast DNA. Recombinant plasmids were constructed from fragments produced by partial digestion of the chloroplast DNA with EcoRI and the pMB9 plasmid as a vector. Five recombinants containing the 4.5S and 5S genes were selected by the colony hybridization technique. One of these plasmids contained also the 16S and 23S RNA genes and was mapped using several restriction endonucleases as well as DNA-RNA hybridization. The order of rRNA genes is 16S-23S-4.5S-5S and the four rRNA genes are coded for by the same DNA strand. |
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Keywords: | Ribosomal DNA pMB9 plasmid physical map coding strand |
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