Effect of Magnesium tanshinoate B on the production of nitric oxide in endothelial cells |
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Authors: | O Karmin Filly Cheung Fion L Sung Da-Yuan Zhu Yaw L Siow |
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Institution: | (1) Department of Pharmacology, Faculty of Medicine, University of Hong Kong, Hong Kong, China;(2) Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China |
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Abstract: | Nitric oxide (NO) is a potent vasodilator which plays an important role in regulating vascular tones. Danshen, a Chinese herbal medicine has been widely used for the treatment of cardiovascular diseases. The objective of this study was to investigate the effect of magnesium tanshinoate B (MTB), a compound purified from Danshen, on the production of NO in human endothelial cell line (ECV304). After cells were incubated with MTB (1-10 µM) for 1 or 4 h, amounts of NO metabolites released by cells were quantified and cellular NOS activities were determined following the conversion of 3H]arginine to 3H]citrulline. The NOS protein expression was determined by Western immunoblotting analysis. MTB (1-10 µM) stimulated the release of NO and its metabolites from endothelial cells. Following MTB treatment, the cellular NOS activities were significantly enhanced with a concomitant increase in the levels of constitutive NOS (cNOS) protein mass (110-178%). Selective activation of cNOS by MTB may be employed therapeutically in modulating NO production in endothelial cells. |
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Keywords: | nitric oxide endothelial cells Salviae miltiorrhizae magnesium tanshinoate B |
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