Exercise training changes the gating properties of large-conductance Ca2+-activated K+ channels in rat thoracic aorta smooth muscle cells |
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| Authors: | Hu-cheng Zhao Fa Wang |
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| Affiliation: | 1. Instituto de Física Rosario, FCEIA-UNR-CONICET, Bv. 27 de Febrero 210 bis, S2000EZP Rosario, Argentina;2. Departamento de Engenharia de Materiais — Universidade Federal de São Carlos, Rodovia Washington Luís, km 235 — SP-310, São Carlos, SP 13565-905, Brazil;3. Institut für Werkstoffkunde und Werkstofftechnik, TU Clausthal, Agricolastr.6, 38678 Clausthal-Zellerfeld. Helmholtz-Zentrum Geesthacht, GEMS Outstation, Notkestr. 85, 22607 Hamburg, Germany;1. Helmerich Advanced Technology Research Center, Oklahoma State University, Tulsa, OK 74106, USA;2. Institute of Materials and Manufacturing, Brunel University London, Uxbridge, London UB8 3PH, UK;3. Brunel Institute for Bioengineering, Brunel University London, Uxbridge, London UB8 3PH, UK;4. Electrical and Computer Engineering Department, North Carolina State University, Raleigh, NC 27606, USA;5. Biomaterials and Advanced Drug Delivery Laboratory, Stanford University, Palo Alto, CA 94305, USA;6. Department of Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, Tulsa, OK 74107, USA;7. Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Palo Alto, CA 94305, USA;8. Stanford Cardiovascular Institute, Stanford University School of Medicine, Palo Alto, CA 94305, USA;9. Department of Developmental Sciences, Marquette University School of Dentistry, Milwaukee, WI 53233, USA;10. Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK |
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| Abstract: | Large-conductance Ca2+-activated K+ (BKCa) channels play a critical role in regulating the cellular excitability in response to change in blood flow. It has been demonstrated that vascular BKCa channel currents in both humans and rats are increased after exercise training. This up-regulation of the BKCa channel activity in arterial myocytes may represent a cellular compensatory mechanism of limiting vascular reactivity to exercise training. However, the underlying mechanisms are not fully understood. In the present study, we examined the single channel activities and kinetics of the BKCa channels in rat thoracic aorta smooth muscle cells. We showed that exercise training significantly increased the open probability (Po), decreased the mean closed time and increased the mean open time, and the sensitivity to Ca2+ and voltage without altering the unitary conductance and the K+ selectivity. Our results suggest a novel mechanism by which exercise training increases the K+ currents by changing the BKCa channel activities and kinetics. |
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