Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes |
| |
| Authors: | BoBae Lee Lin Han Eliot H Frank Susan Chubinskaya Christine Ortiz Alan J Grodzinsky |
| |
| Institution: | 1. Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA;2. Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA;3. Department of Biochemistry, Rush University Medical Center, Chicago, IL 60612, USA;4. Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA;5. Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA;6. Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA;1. Department of Biomedical Engineering, Lund University, Box 188, 221 00, Lund, Sweden;2. Department of Applied Physics, University of Eastern Finland, Yliopistonranta 1, FI-70210, Kuopio, Finland;1. Materials Science and Engineering Graduate Program, University of California, San Diego, La Jolla, CA, USA;2. Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA;3. Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA;4. Department of Orthopaedic Surgery, University of California, San Diego, La Jolla, CA, USA;5. Center for Musculoskeletal Research, Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA;6. Department of Orthopaedic Surgery, Scripps Clinic, La Jolla, CA, USA;2. Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA;3. Fidia Farmaceutici S.p.A., Padua, Italy;4. Department of Physics, Cornell University, Ithaca, NY, USA;6. Rheumatology Unit, Department of Medicine-DIMED, University of Padua, Padua, Italy;5. Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, USA;1. Department of Applied Physics, University of Eastern Finland, Kuopio FI-70211, Finland;2. Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada;1. Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, United States;2. Department of Materials Science and Engineering, Cornell University, Ithaca, NY, United States;4. Department of Physics, Cornell University, Ithaca, NY, United States;5. Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, United States |
| |
| Abstract: | The success of cell-based tissue engineering approaches in restoring biological function will be facilitated by a comprehensive fundamental knowledge of the temporal evolution of the structure and properties of the newly synthesized matrix. Here, we quantify the dynamic oscillatory mechanical behavior of the engineered matrix associated with individual chondrocytes cultured in vitro for up to 28 days in alginate scaffolds. The magnitude of the complex modulus (|E*|) and phase shift (δ) were measured in culture medium using Atomic Force Microscopy (AFM)-based nanoindentation in response to an imposed oscillatory deformation (amplitude ～5 nm) as a function of frequency (f=1–316 Hz), probe tip geometry (2.5 μm radius sphere and 50 nm radius square pyramid), and in the absence and presence of growth factors (GF, insulin growth factor-1, IGF-1, and osteogenic protein-1, OP-1). |E*| for all conditions increased nonlinearly with frequency dependence approximately f1/2 and ranged between ～1 and 25 kPa. This result, along with theoretical calculations of the characteristic poroelastic relaxation frequency, fp, (～50–90 Hz) suggested that this time-dependent behavior was governed primarily by fluid flow-dependent poroelasticity, rather than flow-independent viscoelastic processes associated with the solid matrix. |E*(f)| increased, (f) decreased, and the hydraulic permeability, k, decreased with time in culture and with growth factor treatment. This trend of a more elastic-like response was thought to be associated with increased macromolecular biosynthesis, density, and a more mature matrix structure/organization. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
