8-Hydroxy-5-deazaflavin-dependent electron transfer in the extreme halophile Halobacterium cutirubrum |
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Authors: | LEA de Wit APM Eker |
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Institution: | Biochemical and Biophysical Laboratory, Delft University of Technology, Delft, The Netherlands |
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Abstract: | Abstract Cell extracts of the extreme halophile Halobacterium cutirubrum were found to contain 8-hydroxy-5-deazaflavin as well as 8-OH-5-deazaflavin: NADPH oxidoreductase activity. The oxidoreductase was partially purified and showed maximum activity at pH 5.4, which is unusually low for halobacteria, and 5.3 M NaCl, close to the intracellular salt concentration. The results indicate the presence of an 8-OH-5-deazaflavin-dependent electron transfer system in a nonmethanogenic organism. |
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Keywords: | Halobacterium cutirubrum Halophile extreme 8-Hydroxy-5-deazaflavin (Electron transfer) |
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