Degradation of chloroform by immobilized cells of <Emphasis Type="Italic">Bacillus</Emphasis> sp. in calcium alginate beads |
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Authors: | Kaushik?Dey Email author" target="_blank">Pranab?RoyEmail author |
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Institution: | (1) Department of Biotechnology, University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, India;(2) Durgapur College of Commerce and Science, Rajbandh, Durgapur, Burdwan, 713212, West Bengal, India |
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Abstract: | A Bacillus sp., capable of degrading chloroform, was immobilized in calcium alginate. The beads in 20 g alginate l−1 (about 2 × 108 cells/bead) could be re-used nine times for degradation of chloroform at 40 μM. The immobilized cells had a higher range
of tolerance (pH 6.5–9 and 20–41°C) than free cells (pH 7–8.5 and 28–32°C). At 5 g alginate l−1, leakage of the cells from the beads was 0.51 mg dry wt ml−1. This species is the first reported Bacillus that can degrade chloroform as the sole carbon source. |
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