LC-NMR and LC-MS analysis of 2,3,10,11-oxygenated protoberberine metabolites in Corydalis cell cultures |
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| Authors: | Iwasa Kinuko Kuribayashi Ayako Sugiura Makiko Moriyasu Masataka Lee Dong-Ung Wiegrebe Wolfgang |
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| Institution: | Kobe Pharmaceutical University, 4-19-1 Motoyamakita, Higashinada-ku, Kobe 658-8558, Japan. k-iwasa@kobepharma-u.ac.jp |
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| Abstract: | The metabolism of 2,3,10,11-oxygenated protoberberine alkaloids was studied in cell cultures of Corydalis species. Without prior isolation, the structures of the metabolites were determined by LC-MS and LC-NMR analyses. Tetrahydropseudocoptisine alpha-N-metho salt, pseudoprotopine, and pseudomuramine were identified for the first time, and preliminary evidence for metabolic pathways to the formation of these alkaloids were obtained. |
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| Keywords: | Corydalis platycarpa Corydalis ochotensis var raddeana Fumariaceae Cell cultures Biotransformation LC-MS LC-NMR Secondary metabolism Protoberberine alkaloids |
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