Use of electrophoretic enzyme patterns for streptomycete systematics |
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Authors: | Chung-hun Oh Mi-hyun Ahn Jae-heon Kim |
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Affiliation: | Department of Microbiology, College of Natural Sciences, Dan-kook University, Cheon-an, 330-7/4, South Korea; Research Center for Molecular Microbiology. Seoul National Unirersity, Seoul, 151–742, South Korea |
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Abstract: | Abstract The relative electrophoretic mobilities of various enzymes from 24 different streptomycetes were determined on polyacrylamide gels in order to examine the relatedness of species and strains of the genus Streptomyces . Of 11 different enzymes tested in this study, hexokinase, glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, malate dehydrogenase and isocitrate dehydrogenase showed a limited number of constant and reproducible polymorphic enzyme patterns, by comparing which the inter-specific relationships could be examined. In contrast, glucose dehydrogenase, alcohol dehydrogenase, 3-hydroxybutyrate dehydrogenase, phosphoglucose isomerase, peroxidase and esterase exhibited either weak non-reproducible or highly heterogeneous band patterns which were suitable for dissecting the strains within a species and a cluster group. |
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Keywords: | Multilocus enzyme Polymorphic enzyme pattern Taxonomy of Streptomyces |
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