Vanadate increases oxygen affinity and affects enzyme activities and membrane properties of erythrocytes |
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| Authors: | Joan Ll Vives-Corrons Josep M Jou Anna Ester Maria Ibars Josep Carreras Ramon Bartrôns Fernando Climent Santiago Crisolía |
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| Institution: | Laboratori i Escola Professional d''Hematologia Hospital Clínic. Casanova, 143. Barcelona-36 Spain;Department de Bioquímica. Facultat de Medicina Universitat de Barcelona. Casanova, 143. Barcelona-36 Spain;Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia. Amadeo de Saboya, 4. Valencia Spain |
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| Abstract: | Incubation of blood with vanadate markedly increases the affinity of hemoglobin for oxygen, decreases the deformability of erythrocytes, reduces their osmotic fragility and alters their morphology, determining the appearance of equinocytic forms. Since vanadate is easily taken up by the erythrocytes and binds hemoglobin, these effects might result from interactions of vanadate with hemoglobin and with membrane proteins at the glycerate-2, 3-P2 and/or ATP binding site. In addition, vanadate inhibits phosphoglycerate mutase, phosphoglucomutase and adenylate-kinase activities from hemolysates, suggesting a possible inhibitory effect on erythrocyte metabolism |
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