双歧杆菌及肠致病性大肠杆菌粘附的细胞膜通透性研究

采用乳酸脱氢酶释放法比较研究双歧杆菌１０２７株及肠致生大肠杆菌（ＥＰＥＣ）对体外肠上皮细胞Ｌｏｖｏ细胞株粘附的细胞膜通透性，探讨它们对肠上皮细胞的不同生物学交谈２。结果表明，双歧杆菌粘附Ｌｏｖｏ细胞后，宿主细胞释放ＬＤＨ远较ＥＰＥＣ粘附的效果低，提示双歧杆菌粘附对主细胞膜通生影响不大，而ＥＥＣ则可损伤宿主细胞膜而增加其通性。因此，双歧杆菌作为生理性细菌可与肠上皮细胞和谐共生，这与ＥＰＥＣ的粘附损伤

COMPARISON RESEARCH OF PERMEABILITY OF INTESTINAL EPITHELIAL CELLS MEMBRANE AFTER ADHESION OF BIFIDOBACTERIUM 1027 STRAIN OR ENTEROPATHOGENIC E.COLI

LDH release assay was used to study the changes of extracellular LDH of Lovo cells infected with bifidobacterium 1027 strain or enteropathogenic Escherichia coli(EPEC),which indicated host cells membrane permeability. The results showed that bifidobacterium 1027 strsin could induce only slightly elevation of extracellular LDH of cultured Lovo cells, but EPEC could induce remarkable changes of extracellular LDH of host cells, which suggested elevation of membrane permeability of host cells by adherence of EPEC to Lovo cells. We propose that bifidobacteria acting as phisiological microorganism do no harm to epithelial cells, but EPEC can damage host cells after EPEC adhere to epithilial cells.