Fatty Acid Profiles of Erwinia amylovora as Influenced by Growth Medium, Physiological Age and Experimental Conditions |
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| Authors: | F. Casano J. Wells T. van der Zwet |
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| Affiliation: | Istituto Sperimentale per la Patologia Vegetale, Rome, Italy;U.S. Dept. of Agriculture, ARS, Cook College, Rutgers University, New Brunswick, N.J., U.S.A.;U.S. Dept. of Agriculture, ARS, Appalachian Fruit Research Station, Kearneysville, W. Va., U.S.A. |
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| Abstract: | Total cellular fatty acids for 20 strains of Erwinia amylovora grown on trypticase soy agar (TSA) for 3 days at 27°C, and for 3 strains grown on nutrient agar (NA), King's B (KB), glucoseyeast extract carbonate (GYCA) and TSA for 1, 3 and 6 days were analyzed by gas-liquid chromatography. Forty one percent of total fatty acids were saturated even-carbon straight chains, 6 % were saturated odd-carbon chains, 34 % unsaturated acids, 6 % hydroxy-substituted acids, 1 % branched chains, 7 % cyclic acids, and 4 % unidentified components. Composition was affected by growth medium, physiological age of cells, and chromatograph sensitivity. On TSA and NA saturated odd-carbon and cyclic acids of the 3 strains (combining 1, 3 and 6-day data) were higher than on KB and GYCA. Cyclic acids increased with physiological age on GYCA and KB medium. Even-carbon straight chain and unsaturated fatty acids (major components) constituted a significantly lower percentage of total fatty acids in chromatograms of high sensitivity (30–50 components) than of low instrument sensitivity (15–20 components). |
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