An improved amperometric immunosensor for the detection and enumeration of protein A-bearing Staphylococcus aureus |
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Authors: | B. Mirhabibollahi Joy. L. Brooks R.G. Kroll |
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Affiliation: | Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, UK. |
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Abstract: | An amperometric immunosensor specific to the protein A of Staphylococcus aureus, was developed using the direct electrochemical detection of phenol produced by alkaline phosphatase from phenylphosphate. The immunosensor could detect protein A at 0.01 ng/ml and could reliably detect and quantify pure cultures of protein A-bearing Staph. aureus above 10(3) cfu/ml. A similar sensitivity of detection was obtained with samples of beef and milk. |
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