An improved amperometric immunosensor for the detection and enumeration of protein A-bearing Staphylococcus aureus |
| |
Authors: | B Mirhabibollahi Joy L Brooks RG Kroll |
| |
Institution: | Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, UK. |
| |
Abstract: | An amperometric immunosensor specific to the protein A of Staphylococcus aureus, was developed using the direct electrochemical detection of phenol produced by alkaline phosphatase from phenylphosphate. The immunosensor could detect protein A at 0.01 ng/ml and could reliably detect and quantify pure cultures of protein A-bearing Staph. aureus above 10(3) cfu/ml. A similar sensitivity of detection was obtained with samples of beef and milk. |
| |
Keywords: | |
|
|