Functional characterisation of human SGLT-5 as a novel kidney-specific sodium-dependent sugar transporter |
| |
Authors: | Grempler Rolf Augustin Robert Froehner Stefanie Hildebrandt Tobias Simon Eric Mark Michael Eickelmann Peter |
| |
Affiliation: | Department of CardioMetabolic Diseases Research, Boehringer Ingelheim Pharma GmbH & Co.KG, Biberach, Germany. rolf.grempler@boehringer-ingelheim.com |
| |
Abstract: | Sodium glucose cotransporters (SGLT) actively catalyse carbohydrate transport across cellular membranes. Six of the 12 known SGLT family members have the capacity to bind and/or transport monosaccharides (SGLT-1 to 6); of these, all but SGLT-5 have been characterised. Here we demonstrate that human SGLT-5 is exclusively expressed in the kidney. Four splice variants were detected and the most abundant SGLT-5-mRNA was functionally characterised. SGLT-5 mediates sodium-dependent [(14)C]-α-methyl-D-glucose (AMG) transport that can be inhibited by mannose, fructose, glucose, and galactose. Uptake studies using demonstrated high capacity transport for mannose and fructose and, to a lesser extent, glucose, AMG, and galactose. SGLT-5 mediated mannose, fructose and AMG transport was weakly (μM potency) inhibited by SGLT-2 inhibitors. In summary, we have characterised SGLT-5 as a kidney mannose transporter. Further studies are warranted to explore the physiological role of SGLT-5. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|