Surface plasmon resonance assay for chloramphenicol without surface regeneration |
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Authors: | Jing Yuan James Addo Yinqiu Wu |
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Affiliation: | a Biosensors and Biomeasurement, New Zealand Institute for Plant and Food Research, Hamilton, Waikato 2001, New Zealand b Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia |
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Abstract: | A surface plasmon resonance (SPR) assay without surface regeneration was developed for rapid and sensitive detection of chloramphenicol (CAP). A CAP-amine derivative was synthesized using a polyethylene glycol chain attached to the CAP through a carbamate linkage and immobilized onto a Biacore dextran surface. This chemically modified surface significantly changed the binding behavior between antibody and CAP, shown by both fast association and fast dissociation rates, and created a rapid and sensitive SPR immunoassay of the CAP without any regeneration. The limits of detection achieved for CAP were 32.2 pg/ml in aqueous buffer and 42.4 pg/ml in honey-spiked samples. |
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