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Application of retrovirus-mediated expression cloning for receptor screening of a parasite |
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| Authors: | Kyousuke Kobayashi Tatsuki Sugi Masayuki Shimojima Hiroomi Akashi |
| Affiliation: | a Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan b Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan |
| Abstract: | Retrovirus-mediated expression cloning has been applied in both virology and cell biology. Although there is some difficulty in applying this technique to screening for a receptor recognized by an intracellular parasite, we modified the conventional method to identify a putative receptor for the Plasmodium falciparum BAEBL protein. We show that this method is effective in screening for a parasite receptor. |
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