Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells |
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Authors: | Opitz Christiane A Rimmerman Neta Zhang Yanmin Mead Laura E Yoder Mervin C Ingram David A Walker J Michael Rehman Jalees |
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Institution: | Krannert Institute of Cardiology, Indiana University School of Medicine, 1800 North Capitol Avenue, Room E310, Indianapolis, IN, United States. |
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Abstract: | Recent studies have highlighted the importance of paracrine growth factors as mediators of pro-angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid-based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2-arachidonoylglycerol by distinct human EPC sub-types was measured using HPLC/tandem mass-spectrometry. Anandamide release was highest by adult blood colony-forming EPCs at baseline and they also demonstrated increased 2-arachidonoylglycerol release with TNF-alpha stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro-inflammatory adhesion molecule CD106 (VCAM-1) by TNF-alpha. |
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Keywords: | Endothelium-derived factor Inflammation Endothelium Endothelial progenitor cell Endocannabinoid |
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