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Production of a fructosyl-transferring enzyme byAureobasidium sp. ATCC 20524
Authors:Dr Sachio Hayashi  Masaharu Nonokuchi  Kiyohisa Imada  Hideo Ueno
Institution:(1) Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University, 1-1 Nishi, Gakuen Kibanadai, 889-21 Miyazaki, Japan;(2) Nippon Oligo Corporation, Izumisawa, Toyama, Japan
Abstract:Summary Production of a fructose-transferring enzyme byAureobasidium sp. ATCC 20524 and reaction conditions for the production of fructooligosaccharide, isokestose, were studied. The maximum total enzymatic activity of culture broth was 103.2 U/ml. The optimum reation pH and temperature of intracellular enzyme was 5–6 and 50°C, respectively.
Keywords:Fructooligosaccharide  Industrial microorganism  Isokestose  Reaction condition  Sucrosehydrolyzing enzyme
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