Production of a fructosyl-transferring enzyme byAureobasidium sp. ATCC 20524 |
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Authors: | Dr Sachio Hayashi Masaharu Nonokuchi Kiyohisa Imada Hideo Ueno |
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Institution: | (1) Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University, 1-1 Nishi, Gakuen Kibanadai, 889-21 Miyazaki, Japan;(2) Nippon Oligo Corporation, Izumisawa, Toyama, Japan |
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Abstract: | Summary Production of a fructose-transferring enzyme byAureobasidium sp. ATCC 20524 and reaction conditions for the production of fructooligosaccharide, isokestose, were studied. The maximum total enzymatic activity of culture broth was 103.2 U/ml. The optimum reation pH and temperature of intracellular enzyme was 5–6 and 50°C, respectively. |
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Keywords: | Fructooligosaccharide Industrial microorganism Isokestose Reaction condition Sucrosehydrolyzing enzyme |
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