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Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map |
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| Authors: | Tamilla Nechiporuk Alex Nechiporuk Xiaoping Guan Richard Frederick Karla Figueroa Ilya Chumakov Julie R Korenberg Pieter J de Jong Stefan M Pulst |
| Institution: | (1) Division of Neurology, Cedars-Sinai Medical Center, University of California at Los Angeles, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA, Fax: (310) 652-2465, US;(2) Department of Medical Genetics, Cedars-Sinai Medical Center, University of California at Los Angeles, 8700 Beverly Boulevard, CA 90048, USA, US;(3) Centre d’Etude du Polymorphisme Humain, 27, Rue Juliette Dodu, F-75010, Paris, France, FR;(4) Department of Human Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263-0001, USA, US |
| Abstract: | Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease recently mapped to chromosome 12q close to the locus D12S84 by genetic linkage analysis. To generate additional genetic markers in the SCA2 region, we constructed a physical map of the region using yeast artificial chomosome (YAC), P1 artificial chromosome (PAC) and cosmid clones. The physical map was found to agree well with the genetic map. Three novel microsatellite markers were isolated and physically mapped. A novel approach to isolate CAG repeats directly from YAC DNAs is described. Received: 25 January 1995 / Revised: 26 September 1995 |
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