Production of a highly asymmetric somatic hybrid between rice and Zizania latifolia (Griseb): evidence for inter-genomic exchange |
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Authors: | B Liu Z L Liu X W Li |
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Institution: | (1) Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China, CN |
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Abstract: | A highly asymmetric and fertile somatic hybrid plant was obtained via protoplast fusion in an intergenric combination. Gamma-ray-irradiated
Zizania latifolia (Griseb). Turcz. ex Stapf mesophyll protoplasts were electrofused with idoacetamide-inactivated rice protoplasts derived
from a 2-month-old suspension cell culture. Two of the six putative hybrid calli regenerated plants. Cytological observation
showed that the somatic chromosome numbers of both plants were the same as the rice parent (2n=24). Nevertheless, the hybrid
nature and inter-genomic exchange events of one of the plants, i.e. SH6 (SH for somatic hybrid), were confirmed by Southern
analysis using both total genomic DNA and moderate-copy, Z. latifolia-abundant DNA sequences as probes; in both cases, parental specific and/or new intergenomic recombinant hybridization fragments
were detected. In both plant and seed morphology, the hybrid (SH6) was distinct from its rice parental cultivar, as well as
from the wild donor species, Z. latifolia.
Received: 15 August 1998 / Accepted: 30 September 1998 |
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Keywords: | Rice Zizania Latifolia (Griseb) Turcz ex Stapf Somatic hybridization Introgression |
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