Molecular cloning of the gene for the beta-lactamase of Bacillus licheniformis and its expression in Escherichia coli |
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Authors: | W J Brammar S Muir A McMorris |
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Institution: | (1) Department of Biochemistry, University of Leicester, University Road, LE1 7RH Leicester, England;(2) Department of Molecular Biology, University of Edinburgh, Mayfield Road, EH9 3JR Edinburgh, Scotland |
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Abstract: | Summary The structural gene, pen, for the -lactamase of B. licheniformis has been cloned into a vector and shown to be expressed at a low rate in E. coli. The cloned pen gene appears to be expressed from a promoter within the fragment of B. licheniformis DNA, since its rate of expression is not affected by the presence of the phage repressor, the absence of the phage's positive-control functions, or the position or orientation of the gene within the phage genome. |
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